The Eps15 homology (EH) domain-based interaction between Eps15 and Hrb connects the molecular machinery of endocytosis to that of nucleocytosolic transport

Citation
M. Doria et al., The Eps15 homology (EH) domain-based interaction between Eps15 and Hrb connects the molecular machinery of endocytosis to that of nucleocytosolic transport, J CELL BIOL, 147(7), 1999, pp. 1379-1384
Citations number
24
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell & Developmental Biology
Journal title
JOURNAL OF CELL BIOLOGY
ISSN journal
0021-9525 → ACNP
Volume
147
Issue
7
Year of publication
1999
Pages
1379 - 1384
Database
ISI
SICI code
0021-9525(199912)147:7<1379:TEH(DI>2.0.ZU;2-1
Abstract
The Eps15 homology (EH) module is a protein-protein interaction domain that establishes a network of connections involved in various aspects of endocy tosis and sorting. The finding that EH-containing proteins bind to Hrb (a c ellular cofactor of the Rev protein) and to the related protein Hrbl raised the possibility that the EH network might also influence the so-called Rev export pathway, which mediates nucleocytoplasmic transfer of proteins and RNAs. In this study, we demonstrate that Eps15 and Eps15R, two EH-containin g proteins, synergize with Hrb and Hrbl to enhance the function of Rev in t he export pathway. In addition, the EH-mediated association between Eps15 a nd Hrb is required for the synergistic effect. The interaction between Eps1 5 and Hrb occurs in the cytoplasm, thus pointing to an unexpected site of a ction of Hrb, and to a possible role of the Eps15-Hrb complex in regulating the stability of Rev.