Novel matrices for biosensor applications by structural design of redox-chromogenic aminocellulose esters

Citation
J. Tiller et al., Novel matrices for biosensor applications by structural design of redox-chromogenic aminocellulose esters, J APPL POLY, 75(7), 2000, pp. 904-915
Citations number
22
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Organic Chemistry/Polymer Science","Material Science & Engineering
Journal title
JOURNAL OF APPLIED POLYMER SCIENCE
ISSN journal
0021-8995 → ACNP
Volume
75
Issue
7
Year of publication
2000
Pages
904 - 915
Database
ISI
SICI code
0021-8995(20000214)75:7<904:NMFBAB>2.0.ZU;2-7
Abstract
The solubility and film forming properties of newly developed cellulose der ivatives with 1,4-phenylenediamine (PDA) substituents at position C6 of the anhydroglucose unit (AGU) can be achieved by formation of acetate, benzoat e, and carbanilate groups, respectively, preferably in position C2/C3. All derivatives, if soluble in N,N-dimethylacetamide (DMA) or dimethylsulfoxide (DMSO), form transparent films from their solutions. The solubility of the se PDA cellulose esters in DMSO or DMA, such as the aging, enzyme immobiliz ation, and redox-chromogenic behaviors of the films, depending on the natur e and the degree of substitution of the functional groups were investigated . Regarding the influence of the autoxidation by air-oxygen of the PDA grou ps on the storage stability, the longest stable derivatives were the PDA ce llulose carbanilates. Onto PDA cellulose derivate films glucose oxidase was immobilized via diazo coupling, and by glutardialdehyde and ascorbic acid reactions with an obtained enzyme activity of 45 (diazo coupling, PDA cellu lose acetate) to 145 mU/cm(2) (ascorbic acid, PDA cellulose carbanilate). T he redox-chromogenic properties were demonstrated by oxidative coupling rea ction of typical reagents like phenol and chinoline derivatives onto the PD A groups of the cellulose esters in presence of H2O2 and peroxidase. The be st coloring results were obtained by using PDA cellulose carbanilates und b enzoates. (C) 2000 John Wiley & Sons, Inc.