Clarithromycin and amoxicillin susceptibility of Helicobacter pylori strains isolated from adult patients with gastric or duodenal ulcer in Italy

Franzin, L Pennazio, M Cabodi, D Rossini, FP Gioannini, P

L. Franzin et al., Clarithromycin and amoxicillin susceptibility of Helicobacter pylori strains isolated from adult patients with gastric or duodenal ulcer in Italy, CURR MICROB, 40(2), 2000, pp. 96-100

33

INGLESE

Article

Microbiology

CURRENT MICROBIOLOGY

0343-8651 → ACNP

40

2

2000

96 - 100

ISI

0343-8651(200002)40:2<96:CAASOH>2.0.ZU;2-8

Helicobacter pylori strains, isolated from 100 gastric biopsies from 49 pre viously untreated adult patients with endoscopy and histology-confirmed gas tric or duodenal ulcer, were tested for in vitro antimicrobial susceptibili ty. Strains were isolated from biopsies of 75.5% (37 of 49) patients before therapy and of 13.5% after therapy. Clarithromycin and amoxicillin suscept ibility testing was performed on pretreatment and posttreatment strains by using the agar disk diffusion method and E-test, a quantitative technique f or the minimal inhibitory concentration (MIC) determination. All strains (n = 53) were susceptible to amoxicillin by the two methods. Three strains of 34 (8.8%) patients were resistant to clarithromycin: two by both methods a nd one by E-test (MIC > 2 mu g/ml). E-test, although more expensive than th e disk diffusion method, is easy to perform and is a reliable method for te sting H. pylori susceptibility to antimicrobial agents in the clinical micr obiology laboratory.