Clarithromycin and amoxicillin susceptibility of Helicobacter pylori strains isolated from adult patients with gastric or duodenal ulcer in Italy

Citation
L. Franzin et al., Clarithromycin and amoxicillin susceptibility of Helicobacter pylori strains isolated from adult patients with gastric or duodenal ulcer in Italy, CURR MICROB, 40(2), 2000, pp. 96-100
Citations number
33
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
CURRENT MICROBIOLOGY
ISSN journal
0343-8651 → ACNP
Volume
40
Issue
2
Year of publication
2000
Pages
96 - 100
Database
ISI
SICI code
0343-8651(200002)40:2<96:CAASOH>2.0.ZU;2-8
Abstract
Helicobacter pylori strains, isolated from 100 gastric biopsies from 49 pre viously untreated adult patients with endoscopy and histology-confirmed gas tric or duodenal ulcer, were tested for in vitro antimicrobial susceptibili ty. Strains were isolated from biopsies of 75.5% (37 of 49) patients before therapy and of 13.5% after therapy. Clarithromycin and amoxicillin suscept ibility testing was performed on pretreatment and posttreatment strains by using the agar disk diffusion method and E-test, a quantitative technique f or the minimal inhibitory concentration (MIC) determination. All strains (n = 53) were susceptible to amoxicillin by the two methods. Three strains of 34 (8.8%) patients were resistant to clarithromycin: two by both methods a nd one by E-test (MIC > 2 mu g/ml). E-test, although more expensive than th e disk diffusion method, is easy to perform and is a reliable method for te sting H. pylori susceptibility to antimicrobial agents in the clinical micr obiology laboratory.