Elevated tissue factor levels in leukemic cell homogenate

Citation
T. Nakasaki et al., Elevated tissue factor levels in leukemic cell homogenate, CL APPL T-H, 6(1), 2000, pp. 14-17
Citations number
22
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Hematology
Journal title
CLINICAL AND APPLIED THROMBOSIS-HEMOSTASIS
ISSN journal
1076-0296 → ACNP
Volume
6
Issue
1
Year of publication
2000
Pages
14 - 17
Database
ISI
SICI code
1076-0296(200001)6:1<14:ETFLIL>2.0.ZU;2-3
Abstract
Tissue factor (TF) antigen and activity were measured in leukemic cell homo genates. In leukemic cell homogenate, especially that of acute promyelocyti c leukemia (APL), both TF antigen and activity were significantly higher th an these levels in the mononuclear cells obtained from healthy volunteers. Both TF antigen and activity were significantly higher in myelocytic leukem ia than in lymphocytic leukemia cells. In leukemic cell homogenates, there was a close correlation between TF antigen and TF activity. The TF activity /TF antigen ratio was significantly higher in myelocytic leukemia than in l ymphocytic leukemia cells. As the TF activity was not increased in lymphocy tic leukemia cell homogenates to which were added phospholipids, the decrea se in TF activity in lymphocytic leukemia might not be due to phospholipid in the leukemic cell membrane. Values for TF activity, TF antigen, and the TF activity/TF antigen ratio in leukemic cell homogenate from patients with disseminated intravascular coagulation (DIC) were significantly higher tha n these in patients without DIG. Therefore, the measurement of TF antigen a nd activity in leukemic cells could be useful for the prediction of DIG.