Expression of the mRNA for two isoforms of neural plakophilin-related arm-repeat protein/delta-catenin in rodent neurons and glial cells

Citation
Y. Kawamura et al., Expression of the mRNA for two isoforms of neural plakophilin-related arm-repeat protein/delta-catenin in rodent neurons and glial cells, NEUROSCI L, 277(3), 1999, pp. 185-188
Citations number
16
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences & Behavoir
Journal title
NEUROSCIENCE LETTERS
ISSN journal
0304-3940 → ACNP
Volume
277
Issue
3
Year of publication
1999
Pages
185 - 188
Database
ISI
SICI code
0304-3940(199912)277:3<185:EOTMFT>2.0.ZU;2-Y
Abstract
Neural plakophilin-related arm-repeat protein (NPRAP) is a-mammalian brain protein of the armadillo family. Here, in an attempt to elucidate its funct ion, we determined the mouse brain regions and cell types expressing the mR NAs for two NPRAP isoforms (the longer and the shorter isoforms), and exami ned the regulation of expression of the NPRAP mRNAs during the differentiat ion of P19 cells into neurons. Reverse transcription-polymerase chain react ion (RT-PCR) analysis revealed that both variants were expressed in various mouse brain regions, but the mRNA for the short isoform was predominant in most regions. Primary cultures of both neurons and glial cells exhibited h igh expression levels of both the mRNAs, indicating that NPRAP mRNA express ion is not neuron-specific. The expression of the two NPRAP mRNA variants w as dramatically induced even prior to the terminal neuronal and glial diffe rentiation of P19 cells after retinoic acid treatment. These data suggest t hat the two NPRAP isoforms function not only in neurons and glial cells in the brain, but also play a role in the differentiation of precursor cells i nto neurons and glial cells, (C) 1999 Elsevier Science Ireland Ltd. All rig hts reserved.