Differential expression of inducible nitric oxide synthase gene by alveolar and peritoneal macrophages in lipopolysaccharide-hyporesponsive C3H/HeJ mice

Citation
Mj. Wang et al., Differential expression of inducible nitric oxide synthase gene by alveolar and peritoneal macrophages in lipopolysaccharide-hyporesponsive C3H/HeJ mice, IMMUNOLOGY, 98(4), 1999, pp. 497-503
Citations number
34
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology
Journal title
IMMUNOLOGY
ISSN journal
0019-2805 → ACNP
Volume
98
Issue
4
Year of publication
1999
Pages
497 - 503
Database
ISI
SICI code
0019-2805(199912)98:4<497:DEOINO>2.0.ZU;2-C
Abstract
In lipopolysaccharide (LPS)-hyporesponsive C3H/HeJ mice, alveolar macrophag es (AM phi) produce much more tumour necrosis factor-alpha than peritoneal macrophages (PM phi) when stimulated with LPS (10 mu g/ml), but the inducti on of inducible nitric oxide synthase (iNOS) gene expression and production of nitric oxide (NO) in AM phi are not found. In the present study, we det ermined the induction of iNOS gene expression, using semi-quantitative reve rse transcription-polymerase chain reaction, and the release of NO in AM ph i and PM phi from C3H/HeJ and C3H/HeN mice. The results showed the inductio n of iNOS mRNA accumulation in a dose-dependent manner by LPS alone or in c ombination with interferon-gamma in both macrophages. The effects of the st imuli on iNOS gene expression and NO production were significantly higher i n AM phi than in the PM phi of C3H/HeJ mice. The response of macrophages fr om C3H/HeN mice was similar to those from C3H/HeJ mice, but the difference of iNOS gene expression between AM phi and PM phi in C3H/HeN mice was not a s striking as in C3H/HeJ mice. The results show that the iNOS gene expressi on and NO production were activated differently in AM phi and PM phi and su ggest that the functional properties of macrophages isolated from distinct origins are different.