Increased ornithine decarboxylase activity and DNA synthesis in a cartilage damage model of rat knee joint

Citation
M. Murata et al., Increased ornithine decarboxylase activity and DNA synthesis in a cartilage damage model of rat knee joint, BIOG AMINE, 15(4), 1999, pp. 405-411
Citations number
9
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOGENIC AMINES
ISSN journal
0168-8561 → ACNP
Volume
15
Issue
4
Year of publication
1999
Pages
405 - 411
Database
ISI
SICI code
0168-8561(1999)15:4<405:IODAAD>2.0.ZU;2-D
Abstract
The inductions of ornithine decarboxylase (ODC) activity and DNA synthesis are important events in cartilage healing after damage. ODC catalyzes the r ate-limiting step in the biosynthesis of polyamines that are necessary for cell growth. The purpose of this study was to examine the roles of ODC acti vity and polyamines in the adaptive response of cartilage by using a cartil age damage model of rat knee joint. We measured ODC activity, polyamine lev els and DNA synthesis at 0, 1, 2, 4, 5, 6, 7 days, and 2, 3 and 4 weeks pos t-injury in the cartilage. Cartilage damage resulted in significant increas es in ODC activity,polyamine levels and DNA synthesis in the tissue. Maxima l levels of ODC activity, polyamine contents and DNA synthesis in the carti lage were at day 3, day 4 and day 5, respectively, after the damage. These data suggest that the early increases of cartilage ODC activity and polyami ne levels after the damage are crucial cellular events for the healing of t he cartilage.