Immunohistochemical localization of the differentiation marker E11 in dental development of rats

Citation
W. Schwab et al., Immunohistochemical localization of the differentiation marker E11 in dental development of rats, ACT HISTOCH, 101(4), 1999, pp. 431-436
Citations number
14
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Medical Research Diagnosis & Treatment
Journal title
ACTA HISTOCHEMICA
ISSN journal
0065-1281 → ACNP
Volume
101
Issue
4
Year of publication
1999
Pages
431 - 436
Database
ISI
SICI code
0065-1281(199911)101:4<431:ILOTDM>2.0.ZU;2-G
Abstract
E11 antigen, originally characterized in a rat osteosarcoma cell line, is k nown to be expressed during late stages of the osteogenic cell lineage both in vitro and in vivo. The aim of the present study was to monitor the occu rrence and distribution patterns of the Ell antigen using monoclonal antibo dies (mAb Ell and MEP-1) during different stages of tooth germ development of newborn rats by means of immunohistochemistry. Both antibodies strongly bound to plasma membranes of ameloblasts in presecretory and secretory stag es. In addition, odontoblasts and cells of the periodontium were immunoreac tive for Ell and MEP-I. During maturation, the immunoreactivity of;amelobla st plasma membranes decreased significantly. Our data suggest that Ell and MEP-1 might be important as markers for cell differentiation and mineraliza tion processes during tooth germ development.