Control of its vitro somatic embryogenesis of the spindle tree (Euonymus europaeus L.) by the sugar type and the osmotic potential of the culture medium

Citation
A. Biahoua et L. Bonneau, Control of its vitro somatic embryogenesis of the spindle tree (Euonymus europaeus L.) by the sugar type and the osmotic potential of the culture medium, PL CELL REP, 19(2), 1999, pp. 185-190
Citations number
25
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANT CELL REPORTS
ISSN journal
0721-7714 → ACNP
Volume
19
Issue
2
Year of publication
1999
Pages
185 - 190
Database
ISI
SICI code
0721-7714(199912)19:2<185:COIVSE>2.0.ZU;2-V
Abstract
In vitro somatic embryogenesis was achieved from zygotic embryo explants of a woody angiosperm species, the spindle tree, cultivated on various cultur e media differing in their sugar type and concentration, or in the applied osmotic potential. The highest frequency of somatic embryogenesis was obtai ned with a 350 mM sucrose, or a 89 mM glucose concentration in the culture medium. Experiments with culture media differing only in osmotic potential indicated that a minimal threshold osmotic potential is required to stimula te the emergence of somatic embryos. Elevated concentrations of glucose hav e an inhibitory effect, independent of their osmotic effect, while elevated concentrations of sucrose mainly act osmotically, stimulating the emergenc e of numerous somatic embryos.