Improved formation of regenerable callus in isolated microspore culture ofmaize: impact of carbohydrates, plating density and time of transfer

Citation
M. Nageli et al., Improved formation of regenerable callus in isolated microspore culture ofmaize: impact of carbohydrates, plating density and time of transfer, PL CELL REP, 19(2), 1999, pp. 177-184
Citations number
38
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANT CELL REPORTS
ISSN journal
0721-7714 → ACNP
Volume
19
Issue
2
Year of publication
1999
Pages
177 - 184
Database
ISI
SICI code
0721-7714(199912)19:2<177:IFORCI>2.0.ZU;2-Q
Abstract
Pure fractions of maize (Zea mays L.) microspores at various densities were exposed to defined media containing different concentrations of maltose an d sucrose. In general, lower carbohydrate concentrations (60, 90 g/l) yield ed higher frequencies of embryolike structures than a high concentration (1 20 g/l). Optimum cell density seemed to depend on the genotype, but densiti es above 80,000 microspores/ml led to reduced embryogenesis in all genotype s tested. Direct comparison of maltose and sucrose as carbohydrate source i n the induction medium clearly demonstrated the superiority of maltose with regard to the regeneration frequency. For two out of three genotypes teste d, maltose also enhanced the formation of embryo-like structures. The time of embryo transfer to callus induction media had a significant effect on re generation frequency.