Germination and plantlet regeneration from encapsulated somatic embryos ofmango (Mangifera indica L.)

Citation
H. Ara et al., Germination and plantlet regeneration from encapsulated somatic embryos ofmango (Mangifera indica L.), PL CELL REP, 19(2), 1999, pp. 166-170
Citations number
20
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANT CELL REPORTS
ISSN journal
0721-7714 → ACNP
Volume
19
Issue
2
Year of publication
1999
Pages
166 - 170
Database
ISI
SICI code
0721-7714(199912)19:2<166:GAPRFE>2.0.ZU;2-I
Abstract
Cotyledonary-stage somatic embryos (3-5 mm in length) originating from nuce llar explants of Mangifera indica L. cv. Amrapali were encapsulated individ ually in 2% alginate gel. The encapsulated somatic embryos (ESEs) germinate d successfully on 0.6% agar-gelled medium containing B5 macrosalts (half st rength), Murashige and Skoog microsalts (full strength), 3% sucrose and 2.9 mu M gibberellic acid. The percentage of germination of ESEs was higher th an that of naked somatic embryos of the same size on the same medium. The g erminability of ESEs was increased (73.61+/-7.08%) when the medium was supp lemented with full-strength B5 macrosalts. Of the germinating ESEs, 45.83+/ -3.40% developed into plantlets. Abscisic acid at 0.004 and 0.02 mu M had n o significant influence on germination and plantlet development, but caused a 3-week delay in germination. Well-developed plantlets regenerated from E SEs have been successfully established in soil.