Cryopreservation of in vitro-grown axillary shoot-tip meristems of mint (Mentha spicata L.) by encapsulation vitrification

Authors
Citation
D. Hirai et A. Sakai, Cryopreservation of in vitro-grown axillary shoot-tip meristems of mint (Mentha spicata L.) by encapsulation vitrification, PL CELL REP, 19(2), 1999, pp. 150-155
Citations number
21
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANT CELL REPORTS
ISSN journal
0721-7714 → ACNP
Volume
19
Issue
2
Year of publication
1999
Pages
150 - 155
Database
ISI
SICI code
0721-7714(199912)19:2<150:COIVAS>2.0.ZU;2-L
Abstract
Alginate-coated meristems from in vitro-grown axillary buds of mint (Mentha spicata L.) were successfully cryopreserved by vitrification. Excised meri stems from nodal segments cold hardened at 4 degrees C for 3 weeks were enc apsulated and osmoprotected by a mixture of 2 M glycerol plus 0.4 M sucrose . These meristems were dehydrated with a highly concentrated vitrification solution (PVS2 solution) for 3h at 0 degrees C prior to a plunge into liqui d nitrogen. Successfully encapsulated vitrified meristems developed shoots within a week after plating without intermediary callus formation. The aver age rate of shoot formation amounted to nearly 90%. This procedure was succ essfully applied to other Mentha species. It was also confirmed that encaps ulated vitrified meristems produced a much higher rate of shoot formation t han the encapsulated dried meristems. Thus, this revised encapsulation vitr ification method appears promising for the cryopreservation of mint and oth er germplasm.