Direct somatic embryogenesis on leaf explants of Oncidium Gower Ramsey andsubsequent plant regeneration

Citation
Jt. Chen et al., Direct somatic embryogenesis on leaf explants of Oncidium Gower Ramsey andsubsequent plant regeneration, PL CELL REP, 19(2), 1999, pp. 143-149
Citations number
15
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANT CELL REPORTS
ISSN journal
0721-7714 → ACNP
Volume
19
Issue
2
Year of publication
1999
Pages
143 - 149
Database
ISI
SICI code
0721-7714(199912)19:2<143:DSEOLE>2.0.ZU;2-R
Abstract
Segments taken from young leaves of an orchid (Oncidium Cower Ramsey) produ ced clusters of somatic embryos directly from epidermal and mesophyll cells of leaf tips and wound surfaces without an intervening callus within 1 mon th when cultured on a Gelrite(TM)-gelled 1/2-MS basal medium supplemented w ith a low dosage (0.3-1 mg/l) of thidiazuron. Subculturing of these embryo clusters produced more embryos and subsequent plantlet formation on the sam e medium. The high-frequency embryogenesis of these leaf cells in this orch id is strong evidence of their totipotency, and further modification of the protocol for plant formation could be useful for the mass propagation and transformation of selected elite lines.