Regeneration of fertile plants from isolated zygotes of rice (Oryza sativa)

Citation
J. Zhang et al., Regeneration of fertile plants from isolated zygotes of rice (Oryza sativa), PL CELL REP, 19(2), 1999, pp. 128-132
Citations number
12
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANT CELL REPORTS
ISSN journal
0721-7714 → ACNP
Volume
19
Issue
2
Year of publication
1999
Pages
128 - 132
Database
ISI
SICI code
0721-7714(199912)19:2<128:ROFPFI>2.0.ZU;2-G
Abstract
A simple mechanical method has been developed which allows the routine isol ation of unfertilized and fertilized egg cells from ovules of Japonica and Indica rice varieties. In the experiments described, the majority of the eg g cells and zygotes survived the isolation procedure when the donor plants were in a vigorous state. About 40% of the surviving zygotes underwent sust ained development when cultured in Millicell inserts with a non-morphogenic rice feeder-cell culture. Nearly all zygote-derived callus cultures regene rated multiple shoots, which could be subsequently rooted with high efficie ncy. Zygote-derived plantlets matured to fertile plants when transplanted t o soil. So far, about 80 independent plants each from the Japonica variety 'Taipei309' and the Indica variety 'IR58' have been regenerated. The potent ial of this single-cell regeneration system for marker gene-free transforma tion is discussed.