The effect of germination conditions on the morphology of Coffea arabica L.
somatic embryos mass-produced in a 1-l temporary immersion bioreactor (RIT
A(R)) was studied with emphasis on direct sowing in soil. Using germinated
embryos, direct sowing resulted in a highly successful conversion of embryo
s into plants. A culture density above 1600 embryos per 1-l bioreactor posi
tively affected embryo morphology by causing higher embryonic axis elongati
on (Jr 4-5 mm). At this density, the addition of a high concentration of su
crose (234 mM) 2 weeks before sowing promoted an increase in effective plan
t conversion in soil (78%) and a Vigorous vegetative growth of the resultin
g plants. Furthermore, direct sowing reduced handling time to 13% and shelv
ing area requirements to 6.3% of the values obtained by conventional acclim
atization of plants developed on gel media.