Direct sowing of Coffea arabica somatic embryos mass-produced in a bioreactor and regeneration of plants

Citation
D. Etienne-barry et al., Direct sowing of Coffea arabica somatic embryos mass-produced in a bioreactor and regeneration of plants, PL CELL REP, 19(2), 1999, pp. 111-117
Citations number
22
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANT CELL REPORTS
ISSN journal
0721-7714 → ACNP
Volume
19
Issue
2
Year of publication
1999
Pages
111 - 117
Database
ISI
SICI code
0721-7714(199912)19:2<111:DSOCAS>2.0.ZU;2-X
Abstract
The effect of germination conditions on the morphology of Coffea arabica L. somatic embryos mass-produced in a 1-l temporary immersion bioreactor (RIT A(R)) was studied with emphasis on direct sowing in soil. Using germinated embryos, direct sowing resulted in a highly successful conversion of embryo s into plants. A culture density above 1600 embryos per 1-l bioreactor posi tively affected embryo morphology by causing higher embryonic axis elongati on (Jr 4-5 mm). At this density, the addition of a high concentration of su crose (234 mM) 2 weeks before sowing promoted an increase in effective plan t conversion in soil (78%) and a Vigorous vegetative growth of the resultin g plants. Furthermore, direct sowing reduced handling time to 13% and shelv ing area requirements to 6.3% of the values obtained by conventional acclim atization of plants developed on gel media.