Q beta bacteriophage photoinactivated by methylene blue plus light involves inactivation of its genomic RNA

Citation
Je. Schneider et al., Q beta bacteriophage photoinactivated by methylene blue plus light involves inactivation of its genomic RNA, PHOTOCHEM P, 70(6), 1999, pp. 902-909
Citations number
45
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
PHOTOCHEMISTRY AND PHOTOBIOLOGY
ISSN journal
0031-8655 → ACNP
Volume
70
Issue
6
Year of publication
1999
Pages
902 - 909
Database
ISI
SICI code
0031-8655(199912)70:6<902:QBBPBM>2.0.ZU;2-B
Abstract
Methylene blue (MB) is being used as a sensitizer for the photodynamic inac tivation of viral contaminants, including the human immunodeficiency virus, in blood and blood components used in medical treatment. We recently showe d that oxygen-dependent photodynamic in-activation of the RNA bacteriophage Q beta with MB plus light (MB + L) is associated with the formation of 8-o xo-7,8-dihydroguanine, protein carbonyls, RNA-protein crosslinkages and min or amounts of RNA strand breaks. We report herein, with the use of infectio us RNA assays, that the lethal lesions in Q beta phage following MB + L exp osure can be accounted for, and thereby most likely reside in, the RNA comp onent of the phage but that the protein component of the virion contributes to the inactivation, The formation of RNA-protein crosslinkages as the pri mary inactivating type of lesion is put forth as the most probable model of the inactivation mechanism due to the sensitivity with which RNA-protein c rosslinks are formed in response to MB + L exposure and the expectation of the powerful inactivating power of this type of lesion.