A role for Pref-1 and HES-1 in thymocyte development

Citation
M. Kaneta et al., A role for Pref-1 and HES-1 in thymocyte development, J IMMUNOL, 164(1), 2000, pp. 256-264
Citations number
37
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology
Journal title
JOURNAL OF IMMUNOLOGY
ISSN journal
0022-1767 → ACNP
Volume
164
Issue
1
Year of publication
2000
Pages
256 - 264
Database
ISI
SICI code
0022-1767(20000101)164:1<256:ARFPAH>2.0.ZU;2-9
Abstract
T lymphocyte development requires a series of interactions between developi ng thymocytes and thymic epithelial (TE) cells, In this paper we show that TE cells in the developing thymus express Pref-1, a Delta-like cell-surface molecule. In fetal thymus organ cultures (FTOC), thymocyte cellularity was increased by the exogenous dimeric Pref-1 fusion protein, but was reduced by the soluble Pref-1 monomer or anti-Pref-1 Ab, Dimeric Pref-1 in FTOC als o increased thymocyte expression of the HES-1 transcription factor. Thymocy te cellularity was increased in FTOC repopulated with immature thymocytes o verexpressing HES-1, whereas FTOC from HES-l-deficient mice were hypocellul ar and unresponsive to the Pref-1 dimer, We detected no effects of either P ref-1 or HES-1 on developmental choice among thymocyte lineages. These resu lts indicate that Pref-1 expressed by TE cells and HES-1 expressed by thymo cytes are critically involved in supporting thymocyte cellularity.