Involvement of lipoxygenase-dependent production of fatty acid hydroperoxides in the development of the hypersensitive cell death induced by cryptogein on tobacco leaves

Citation
C. Rusterucci et al., Involvement of lipoxygenase-dependent production of fatty acid hydroperoxides in the development of the hypersensitive cell death induced by cryptogein on tobacco leaves, J BIOL CHEM, 274(51), 1999, pp. 36446-36455
Citations number
66
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
JOURNAL OF BIOLOGICAL CHEMISTRY
ISSN journal
0021-9258 → ACNP
Volume
274
Issue
51
Year of publication
1999
Pages
36446 - 36455
Database
ISI
SICI code
0021-9258(199912)274:51<36446:IOLPOF>2.0.ZU;2-3
Abstract
Lipid peroxidation was investigated in relation with the hypersensitive rea ction in cryptogein-elicited tobacco leaves. A massive production of free p olyunsaturated fatty acid (PUFA) hydroperoxides dependent on a 9-lipoxygena se (LOX) activity was characterized during the development of leaf necrosis . The process occurred after a lag phase of 12 h, was accompanied by the co ncomitant increase of 9-LOX activity, and preceded by a transient accumulat ion of LOX transcripts. Free radical-mediated lipid peroxidation represente d 10% of the process. Inhibition and activation of the LOX pathway was show n to inhibit or to activate cell death, and evidence was provided that fatt y acid hydroperoxides are able to mimic leaf necrotic symptoms. Within 24 h , about 50% of leaf PUFAs were consumed, chloroplast lipids being the major source of PUFAs. The results minimize the direct participation of active o xygen species from the oxidative burst in membrane lipid peroxidation. They suggest, furthermore, the involvement of lipase activity to provide the fr ee PUFA substrates for LOX. The LOX-dependent peroxidative pathway, respons ible for tissue necrosis, appears as being one of the features of hypersens itive programmed cell death.