Cellular senescence of a human bladder carcinoma cell line (JTC-32) induced by a normal chromosome 11

Citation
H. Kugoh et al., Cellular senescence of a human bladder carcinoma cell line (JTC-32) induced by a normal chromosome 11, CANC GENET, 116(2), 2000, pp. 158-163
Citations number
35
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Onconogenesis & Cancer Research
Journal title
CANCER GENETICS AND CYTOGENETICS
ISSN journal
0165-4608 → ACNP
Volume
116
Issue
2
Year of publication
2000
Pages
158 - 163
Database
ISI
SICI code
0165-4608(20000115)116:2<158:CSOAHB>2.0.ZU;2-5
Abstract
Human chromosome 11 is expected to carry tumor suppressor genes for a varie ty of human cancers, including bladder carcinoma. To examine the functional role of a putative tumor suppressor gene(s) on this chromosome in the deve lopment of bladder carcinoma, we performed microcell-mediated transfer of c hromosome 11 into the bladder carcinoma cell line, JTC-32. Fifteen of 20 co lonies formed by the transfer experiment showed a remarkable change in cell morphology. They flattened and ceased growing, or senesced, prior to 10 po pulation doublings. The presence of transferred chromosome 11-derived fragm ents in the growth-arrested cells was confirmed by PCR-based polymorphism a nalyses. The remaining 5 microcell hybrid clones exhibited a parental cell- like morphology, and presumably escaped from senescence, which was accompan ied by deletions and/or rearrangements of the transferred chromosome 11. On the other hand, a transferred normal chromosome 7 neither changed the cell morphology nor arrested the cell growth. These results support the hypothe sis that chromosome 11 contains a gene or genes which restore the senescenc e program lost during the immortalization process of JTC-32 cells. (C) Else vier Science Inc., 1999. All rights reserved.