Myogenic expression of an injectable protease-resistant growth hormone-releasing hormone augments long-term growth in pigs

Citation
R. Draghia-akli et al., Myogenic expression of an injectable protease-resistant growth hormone-releasing hormone augments long-term growth in pigs, NAT BIOTECH, 17(12), 1999, pp. 1179-1183
Citations number
33
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
NATURE BIOTECHNOLOGY
ISSN journal
1087-0156 → ACNP
Volume
17
Issue
12
Year of publication
1999
Pages
1179 - 1183
Database
ISI
SICI code
1087-0156(199912)17:12<1179:MEOAIP>2.0.ZU;2-B
Abstract
Ectopic expression of a new serum protease-resistant porcine growth hormone -releasing hormone, directed by an injectable muscle-specific synthetic pro moter plasmid vector (pSP-HV-GHRH), elicits growth in pigs. A single 10 mg intramuscular injection of pSP-HV-GHRH DNA followed by electroporation in t hree week-old piglets elevated serum GHRH levels by twofold to fourfold, en hanced growth hormone secretion, and increased serum insulin-like growth fa ctor-I by threefold to sixfold over control pigs. After 65 days the average body weight of the pigs injected with pSP-HV-GHRH was similar to 37% great er than the placebo-injected controls and resulted in a significant reducti on in serum urea concentration, indicating a decrease in amino acid catabol ism. Evaluation of body composition indicated a uniform increase in mass, w ith no organomegaly or associated pathology.