Gene expression of the hematopoietic cell phosphatase in juvenile myelomonocytic leukemia

Citation
D. Wutz et al., Gene expression of the hematopoietic cell phosphatase in juvenile myelomonocytic leukemia, LEUK LYMPH, 35(5-6), 1999, pp. 491-499
Citations number
28
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Hematology,"Onconogenesis & Cancer Research
Journal title
LEUKEMIA & LYMPHOMA
ISSN journal
1042-8194 → ACNP
Volume
35
Issue
5-6
Year of publication
1999
Pages
491 - 499
Database
ISI
SICI code
1042-8194(199911)35:5-6<491:GEOTHC>2.0.ZU;2-O
Abstract
We analyzed the relative expression of Hematopoietic cell phosphatase (HCP) in mononuclear cells (MNC) of peripheral blood (PB), bone marrow (BIM) and spleen of patients with juvenile myelomonocytic leukemia (JMML) and normal donors. Two regions of HCP with alternative exon skipping of exon 6 or exo n 12 are described. There was no difference in the expression of the amplif ied HCP cDNA regions in MNC of JMML patients compared to normal donors. The two forms of exon skipping were present in unstimulated MNC of JMML patien ts or normal donors. In contrast, phytohemagglutinin (PHA) stimulated MNC o f normal donors, Epstein-Barr Virus (EBV) transformed B-cells of JMML patie nts, BFU-E and CFU-GM derived colonies of JMML patients, and the cell lines K562 and HEL did not or only barely express these two forms of exon skippi ng. These results may indicate that alternative HCP exon skipping may be as sociated with the proliferative state of the cell.