Characterization of spinach chromosomes by condensation patterns and physical mapping of 5S and 45S rDNAs by FISH

Citation
M. Ito et al., Characterization of spinach chromosomes by condensation patterns and physical mapping of 5S and 45S rDNAs by FISH, J AM S HORT, 125(1), 2000, pp. 59-62
Citations number
20
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences
Journal title
JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE
ISSN journal
0003-1062 → ACNP
Volume
125
Issue
1
Year of publication
2000
Pages
59 - 62
Database
ISI
SICI code
0003-1062(200001)125:1<59:COSCBC>2.0.ZU;2-4
Abstract
Molecular cytogenetic techniques and computer-aided karyotyping were applie d to characterize the chromosomes of spinach (Spinacia oleracea L,, 2n = 12 ). Chromosome lengths, arm ratios, and degrees of condensation at prometaph ase chromosomes were analyzed using a software Chromosome Image Analyzing S ystem III (CHIAS III), DNA probes prepared from rice (Oryza sativa L.) rDNA were applied to the spinach chromosomes by the fluorescence in situ hybrid ization (FISH) method. Three 45S rDNA loci were detected at the nucleolar o rganizing region (NOR) of Chromosome 5, and at terminal positions of short arms of Chromosomes 2 and 6, The loci of 5S rDNA were also found at three l ocations. One was at the subtelomeric region of the long arm of Chromosome 2 and the other two were at the proximal region of the long arm of Chromoso me 5, All spinach chromosomes were identified which will provide valuable i nformation for mapping genes on these chromosomes.