UCP2 and UCP3 rise in starved rat skeletal muscle but mitochondrial protonconductance is unchanged

Citation
S. Cadenas et al., UCP2 and UCP3 rise in starved rat skeletal muscle but mitochondrial protonconductance is unchanged, FEBS LETTER, 462(3), 1999, pp. 257-260
Citations number
27
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
FEBS LETTERS
ISSN journal
0014-5793 → ACNP
Volume
462
Issue
3
Year of publication
1999
Pages
257 - 260
Database
ISI
SICI code
0014-5793(199912)462:3<257:UAURIS>2.0.ZU;2-9
Abstract
The relationship between UCP2 and UCP3 expression and mitochondrial proton conductance of rat skeletal muscle was examined. Rats were starved for 24 h and the levels of UCP2 and UCP3 mRNA and UCP3 protein mere determined by N orthern and Western blots. Proton conductance was measured by titrating mit ochondrial respiration rate and membrane potential with malonate, Starvatio n increased UCP2 and UCP3 mRNA levels more than 5-fold and 4-fold, respecti vely, and UCP3 protein levels by 2-fold. However, proton conductance remain ed unchanged. These results suggest either that Northern and Western blots do not reflect the levels of active protein or that these UCPs do not catal yse the basal proton conductance in skeletal muscle mitochondria, (C) 1999 Federation of European Biochemical Societies.