Development of autologous human dermal-epidermal composites based on sterilized human allodermis for clinical use

Citation
Kh. Chakrabarty et al., Development of autologous human dermal-epidermal composites based on sterilized human allodermis for clinical use, BR J DERM, 141(5), 1999, pp. 811-823
Citations number
30
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Dermatology,"da verificare
Journal title
BRITISH JOURNAL OF DERMATOLOGY
ISSN journal
0007-0963 → ACNP
Volume
141
Issue
5
Year of publication
1999
Pages
811 - 823
Database
ISI
SICI code
0007-0963(199911)141:5<811:DOAHDC>2.0.ZU;2-#
Abstract
The aim of this study was to identify a sterilization technique for the pre paration of human allodermis which could be used as a dermal component in w ound healing and as the dermal base for production of dermal-epidermal comp osites for one-stage grafting in patients. We report that it is possible to produce dermal-epidermal composites which perform well in vitro and in viv o using a standard ethylene oxide sterilization methodology. Prevention of ethylene oxide-induced damage to the dermis was achieved using gentle dehyd ration of the skin prior to ethylene oxide sterilization. The issue of whet her viable fibroblasts are required for composite production was examined i n comparative studies using glycerol vs. ethylene oxide sterilized dermis. Where good collagen IV retention was achieved following preparation of acel lular de-epidermized dermis there was no advantage to having fibroblasts pr esent in vitro or in vivo; however, where collagen IV retention was poor or where keratinocytes were initially expanded in culture then there was a si gnificant advantage to introducing fibroblasts to the composites during the ir preparative 10-day period in vitro. The requirement for fibroblasts beca me less evident when composites were grafted on to nude mice, In conclusion , we report a protocol for the successful sterilization of human allodermis to achieve an acellular dermis with good retention of collagen IV. This ac ellular dermis would be appropriate for clinical use as a dermal replacemen t material, It can also be used for the production of dermal-epidermal comp osites using autologous keratinocytes (with or without fibroblasts).