Overview of the role of molecular methods in the diagnosis of malignant lymphomas

Citation
Lj. Medeiros et J. Carr, Overview of the role of molecular methods in the diagnosis of malignant lymphomas, ARCH PATH L, 123(12), 1999, pp. 1189-1207
Citations number
89
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology","Medical Research Diagnosis & Treatment
Journal title
ARCHIVES OF PATHOLOGY & LABORATORY MEDICINE
ISSN journal
0003-9985 → ACNP
Volume
123
Issue
12
Year of publication
1999
Pages
1189 - 1207
Database
ISI
SICI code
0003-9985(199912)123:12<1189:OOTROM>2.0.ZU;2-7
Abstract
Objective.--To review the role of molecular genetics in the diagnosis of ma lignant lymphomas. Data Sources and Study Selection.--Primary research studies and reviews pub lished in the English literature that focus on molecular genetics and malig nant lymphoma, in particular, clonality, chromosomal translocations, tumor suppressor genes, and Hodgkin disease. Data Extraction and Synthesis.--Molecular genetics has an important role in the assessment of malignant lymphomas. Clonality, detected by Southern blo t analysis or the polymerase chain reaction, is helpful for establishing th e diagnosis of lymphoma in lesions with ambiguous morphologic and immunophe notypic findings. Southern blot analysis is the "gold standard" for clonali ty assessment, but the process is labor-intensive and time-consuming. Polym erase chain reaction analysis is more convenient, but a potentially signifi cant false-negative rate exists in the analysis of some antigen receptor ge nes as a result of using consensus primers and the process of somatic hyper mutation. Chromosomal translocations, which result in oncogene activation, occur in many types of B- and T-cell lymphomas, and their detection is help ful in classification as well as in establishing a diagnosis of malignancy. Gene rearrangements and chromosomal translocations also can be used to mon itor minimal residual disease. Tumor suppressor genes, although their analy sis is relatively less useful for diagnosis, are involved in both pathogene sis and tumor progression and will be more important diagnostically as this field continues to expand. Molecular genetic analysis has played a major r ole in improving our understanding of Hodgkin disease. Conclusions.--Molecular genetic tests are currently important ancillary too ls for the diagnosis and classification of malignant lymphomas, and their r ole is likely to increase in the future.