Based on primers designed according to conserved sequences of the NRAMP1 ge
ne, horse genomic DNA was used for amplification, cloning, and sequencing o
f the putative horse orthologue. Sequence alignment revealed high level of
homologies with other mammalian species ranging from 93.1 % (sheep) to 85.1
% (mouse). The horse sequences were identified as exons 3, 4, 5, and 6. Th
e amino acid position corresponding to the site 169 of the gly to asp subst
itution in mice is occupied by glycine in the hone. Differences between Old
Kladruber and Akhal-Teke hones in allelic frequencies of two intronic RFLP
markets of the horse NRAMP1 gene were found.