Papaya (Carica papaya) lysozyme is a member of the family 19 (Basic, classII) chitinases

Citation
T. Subroto et al., Papaya (Carica papaya) lysozyme is a member of the family 19 (Basic, classII) chitinases, J MOL EVOL, 49(6), 1999, pp. 819-821
Citations number
29
Language
INGLESE
art.tipo
Letter
Categorie Soggetti
Biology,"Experimental Biology
Journal title
JOURNAL OF MOLECULAR EVOLUTION
ISSN journal
0022-2844 → ACNP
Volume
49
Issue
6
Year of publication
1999
Pages
819 - 821
Database
ISI
SICI code
0022-2844(199912)49:6<819:P(PLIA>2.0.ZU;2-A
Abstract
The most comprehensive studies on a plant lysozyme (EC 3.2.1.17) are those on the enzyme from papaya (Carica papaya) latex, published in 1967 and 1969 . However, the N-terminal amino acid sequence of five amino acid sequence o f this enzyme, determined by manual Edman degradation, did not allow assign ment to any of the much later-classified families of glycosyl hydrolases. N -Terminal sequence analysis of 22 residues of papaya lysozyme now shows una mbiguously that the enzyme belongs to the family 19 chitinases. It has prop erties similar to those of basic class I chitinases with lysozyme activity, such as cleavage specificity at the C-1 of N-acetylmuramic acid with inver sion of configuration, but as it lacks an N-terminal hevein domain, it shou ld be classified as a class II chitinase.