Up-modulation of interferon-gamma mediates the enhancement of spontanous cytotoxicity in prolactin-activated natural killer cells

Citation
L. Matera et al., Up-modulation of interferon-gamma mediates the enhancement of spontanous cytotoxicity in prolactin-activated natural killer cells, IMMUNOLOGY, 98(3), 1999, pp. 386-392
Citations number
39
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology
Journal title
IMMUNOLOGY
ISSN journal
0019-2805 → ACNP
Volume
98
Issue
3
Year of publication
1999
Pages
386 - 392
Database
ISI
SICI code
0019-2805(199911)98:3<386:UOIMTE>2.0.ZU;2-T
Abstract
Prolactin (PRL) has been shown to participate in lymphocyte activation. In particular, the constitutive natural killer (NK) and the lymphokine-activat ed killer (LAK) cytotoxicity of CD56(+) CD16(+) cells is increased by its p hysiological to supraphysiological concentrations. As PRL has been shown to up-regulate the production of interferon-gamma (IFN-gamma) by peripheral, blood mononuclear cells, we studied its effect on IFN-gamma production by N K cells as a possible mechanism of autocrine activation of cytotoxicity. Re leased and intracellular IFN-gamma, as well as IFN-gamma mRNA expression, w ere increased by pituitary and recombinant human PRL, which stimulated opti mal NK and LAK cytotoxicity. Treatment with blocking anti-IFN-gamma monoclo nal antibody (mAb) selectively affected PRL-increased killing of K562 targe ts, demonstrating that PRL-mediated enhancement of spontaneous cytotoxicity depends, at least in part, on up-regulation of IFN-gamma.