Comparison of phosphodiesterase isozymes in rodent parotid glands

Citation
A. Imai et al., Comparison of phosphodiesterase isozymes in rodent parotid glands, COMP BIOC B, 124(4), 1999, pp. 397-403
Citations number
31
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
ISSN journal
0305-0491 → ACNP
Volume
124
Issue
4
Year of publication
1999
Pages
397 - 403
Database
ISI
SICI code
0305-0491(199912)124:4<397:COPIIR>2.0.ZU;2-1
Abstract
We investigated phosphodiesterase (PDE) isozymes,which hydrolyze cAMP, in r odent parotid glands (mouse, hamster and guinea pig) in order to clarify th e effects of cGMP and Ca/calmodulin on the regulation of cellular cAMP and compared them with those of the rat. More than 80% of the activities were i n the supernatant fractions except for the hamster. The isozymes were fract ionated using Mono Q ion-exchange column. The mouse parotid PDEs consisted of PDE1 (Ca/calmodulin-dependent), PDE2 (cGMP-stimulated), PDE3 (cGMP-inhib ited) and PDE4 (cAMP-specific) similar to those of the rat. PDE3 was not de tected in the hamster. and PDE4 was not detected in the guinea pig. PDE act ivities in the supernatant of the mouse and the hamster were stimulated by cGMP, and that of the guinea pig was stimulated by Ca/calmodulin. These res ults suggest that various PDE isozymes are present in the parotid gland of several species of order Rodentia. There seems to be differences among the species with regard to the PDE isozymes. (C) 1999 Elsevier Science Inc. All rights reserved.