Morphological and functional characteristics of short-term and long-term bone marrow cultures in chronic myelogenous leukemia

Citation
T. Budak-alpdogan et al., Morphological and functional characteristics of short-term and long-term bone marrow cultures in chronic myelogenous leukemia, AM J HEMAT, 62(4), 1999, pp. 212-220
Citations number
44
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Hematology,"Cardiovascular & Hematology Research
Journal title
AMERICAN JOURNAL OF HEMATOLOGY
ISSN journal
0361-8609 → ACNP
Volume
62
Issue
4
Year of publication
1999
Pages
212 - 220
Database
ISI
SICI code
0361-8609(199912)62:4<212:MAFCOS>2.0.ZU;2-S
Abstract
Clonogenic capacity of bone marrow progenitors and stromal layers establish ed from bone marrow of 12 patients with CML and 13 healthy controls were ev aluated. The initial BFU-E and CFU-GM contents were slightly higher in the CML patients (p > 0.05) in contrast to CFU-GEMM. CFU-GEMM was lower in the patients compared to healthy controls (p < 0.001). In long-term cultures, t he number of non-adherent cell population and total clonogenic progenitor c ell content decreased gradually in both groups. Weekly evaluation of stroma l confluency of adherent cells revealed that establishment of adherent stro mal layer was slower in CML patients than in control samples (p < 0.05). At the end of fourth week, the number of samples presenting confluency was 41 .7% in the CML group compared with 92.3% in the controls. The initial CD34 positive cell content of the bone marrow samples was similar in both groups . Although CD34 positive cell number in the adherent stromal layer was well preserved in the control group at the end of 4 weeks, this figure decrease d significantly in the CML group. The numbers of total adherent cells as we ll as the total clonogenic progenitor content of adherent layer were also l ower in the CML group (3.03% vs 98.2%). When normal CD34+ cells were cultur ed on IFN-alpha-treated stromal layer followed by the assessment of the lon g-term culture initiating cells, a reduced capacity to support hemopoietic growth was observed with IFN-alpha-treated normal stroma. This reduction wa s even higher when CML stroma was treated with IFN-a followed by the seedin g of the normal CD34+ cells on this stromal layer (26.9% vs 42.8%). These f indings show that stromal cells are abnormal in CML patients as well as the progenitor cells, and IFN-alpha treatment causes further defects of the st romal cells. (C) 1999 Wiley-Liss, Inc.