THE A-PROTEIN OF THE FILAMENTOUS BACTERIOPHAGE-CF OF XANTHOMONAS-CAMPESTRIS PV CITRI

Authors
Citation
Mk. Yang et Yc. Yang, THE A-PROTEIN OF THE FILAMENTOUS BACTERIOPHAGE-CF OF XANTHOMONAS-CAMPESTRIS PV CITRI, Journal of bacteriology, 179(9), 1997, pp. 2840-2844
Citations number
26
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
ISSN journal
0021-9193
Volume
179
Issue
9
Year of publication
1997
Pages
2840 - 2844
Database
ISI
SICI code
0021-9193(1997)179:9<2840:TAOTFB>2.0.ZU;2-2
Abstract
Filamentous bacteriophages have very strict host specificities. Experi ments were performed to investigate whether the A protein of the filam entous phage Cf, which infects Xanthomonas campestris pv, citri but no t X. campestris pv. oryzae, is involved in determining Cf's host speci ficity. Tile gene encoding the A protein of Cf mas cloned and expresse d in X: campestris pv. citri, The genomic DNA of another filamentous b acteriophage, Xf, which infects X. campestris is pv. oryzae but not X: campestris pv. citri, was then introduced by electroporation into X. campestris pv. citri that had expressed thf A protein of Cf. The proge ny phages thus produced were able to infect both X, campestris pv, ory zae and X. campestris is pv, citri, indicating that the A protein of C f mas incorporated into the viral particles of Xf and conferred upon X f the ability to infect the host of Cf, Inactivation of the A protein gene abolished the infectivity of Cf. The results of this study indica te that the A protein of Cf is responsible for controlling the host sp ecificity of Cf.