Comparison of different methods for plant regeneration and transformation of the legume Galega orientalis Lam. (goat's rue)

Citation
Amc. Collen et Ci. Jarl, Comparison of different methods for plant regeneration and transformation of the legume Galega orientalis Lam. (goat's rue), PL CELL REP, 19(1), 1999, pp. 13-19
Citations number
27
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANT CELL REPORTS
ISSN journal
0721-7714 → ACNP
Volume
19
Issue
1
Year of publication
1999
Pages
13 - 19
Database
ISI
SICI code
0721-7714(199911)19:1<13:CODMFP>2.0.ZU;2-#
Abstract
For the first time, regeneration and transformation have been achieved from the legume Galega orientalis Lam. (goat's rue). Two different regeneration protocols are described, one based on direct shoot induction from meristem s and the other :involving callus induction and shoot induction from callus with the plant growth regulator thidiazuron (TDZ). Different media and exp lants were evaluated. Three different transformation methods were compared: cocultivation with four different Agrobacterium tumefaciens strains, elect roporation of embryos and apical meristems and particle bombardment of embr yos. TDZ-promoted shoot induction on calli from immature embryos gave the b est results. Transformation using this regeneration protocol was most succe ssful with particle bombardment. Stable transformation has yet to be proven .