Tissue-specific, hormonal, and developmental regulation of SCC-LacZ expression in transgenic mice leads to adrenocortical zone characterization

Citation
Mc. Hu et al., Tissue-specific, hormonal, and developmental regulation of SCC-LacZ expression in transgenic mice leads to adrenocortical zone characterization, ENDOCRINOL, 140(12), 1999, pp. 5609-5618
Citations number
73
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Endocrinology, Nutrition & Metabolism
Journal title
ENDOCRINOLOGY
ISSN journal
0013-7227 → ACNP
Volume
140
Issue
12
Year of publication
1999
Pages
5609 - 5618
Database
ISI
SICI code
0013-7227(199912)140:12<5609:THADRO>2.0.ZU;2-P
Abstract
We report here the study of the human CYP11A1 promoter in driving tissue-sp ecific, developmentally and hormonally regulated reporter gene expression. A 4.4-kb fragment containing all known regulatory elements is more efficien t than a short basal promoter fused to an upstream adrenal enhancer in driv ing reporter LacZ gene expression both in cell culture and in transgenic mi ce. The LacZ gene controlled by the 4.4- and 2.3-kb promoters was expressed in the adrenal cortex, testicular Leydig cells, ovarian corpora lutea, and granulosa cells. Transgene expression in the adrenals was stimulated by AC TH, indicating the presence of ACTH-responsive sequence. beta-Galactosidase activity was first detected in the adrenal primordia at 11.5 days postcoit um. Its expression continued throughout all stages of adrenal development i n a pattern similar to that of the endogenous CYP11A1, which was expressed in all zones of the adrenal cortex, but was strongest in the X zone. The X zone grew before puberty but regressed afterward, as did the levels of CYP1 1A1 and LacZ gene expression in the X zone. Our study of the CYP11A1 promot er in transgenic mice led to characterization of the adrenocortical zones.