Cytogenetic characterization of the holocentric chromosomes in the aphids Myzus varians and Myzus cerasi

Citation
D. Bizzaro et al., Cytogenetic characterization of the holocentric chromosomes in the aphids Myzus varians and Myzus cerasi, CARYOLOGIA, 52(1-2), 1999, pp. 81-85
Citations number
24
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology
Journal title
CARYOLOGIA
ISSN journal
0008-7114 → ACNP
Volume
52
Issue
1-2
Year of publication
1999
Pages
81 - 85
Database
ISI
SICI code
0008-7114(199901/06)52:1-2<81:CCOTHC>2.0.ZU;2-F
Abstract
Cytogenetic investigations on the holocentric chromosomes of Myzus varians and Myzus cerasi have been carried out using silver staining and C-banding followed by chromomycin A(3) (CMA(3)) and DAPI staining in order to improve our knowledge of these pest crop species and facilitate their identificati on. In M. varians C-banding pattern is peculiar of each chromosome pair thu s allowing the identification of all homologues and the reconstruction of a reliable karyotype whereas in M. cerasi, C-positive regions result to be l imited to both the telomeric regions of the X chromosomes. In both species, silver staining localizes rDNA genes on one telomere of each X chromosome; such telomeres are the unique brightly fluorescent C-positive regions afte r CMA(3) staining, whereas all other heterochromatic bands result DAPI posi tive.