The correlation between the consumption of amino acids and the production o
f the polypeptide antibiotic gallidermin by Staphylococcus gallinarum Tu 39
28 was investigated by on-line determination of amino acids and pulse exper
iments. A prolonged production phase together with an increase in galliderm
in formation of about 25% was obtained during pulse and fed-batch experimen
ts with the amino acids glutamic acid, glycine, serine and threonine.