Protein transmission during Dean vortex microfiltration of yeast suspensions

Citation
T. Kluge et al., Protein transmission during Dean vortex microfiltration of yeast suspensions, BIOTECH BIO, 65(6), 1999, pp. 649-658
Citations number
21
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY AND BIOENGINEERING
ISSN journal
0006-3592 → ACNP
Volume
65
Issue
6
Year of publication
1999
Pages
649 - 658
Database
ISI
SICI code
0006-3592(199912)65:6<649:PTDDVM>2.0.ZU;2-N
Abstract
Substantially higher rates of protein and fluid volume transport for microf iltration of yeast suspensions were possible with improved hydrodynamics us ing centrifugal fluid instabilities called Dean vortices. Under constant pe rmeate flux operation with suspended yeast cells, a helical module exhibite d 19 times the filtration capacity of a linear module. For feed containing both BSA and beer yeast under constant transmembrane pressure with diafiltr ation, about twice as much protein (BSA and other proteins from cell lysis) was transported out of the feed by the helical module as compared with the linear module. The volumetric permeation flux improvements for the helical over the linear module ranged from 18 to 43% for yeast concentrations up t o 4.5 dry wt %. (C) 1999 John Wiley & Sons, Inc.