Biochemical alterations secondary to disuse atrophy in the rat's serum andlimb tissues

Citation
I. Cohen et al., Biochemical alterations secondary to disuse atrophy in the rat's serum andlimb tissues, ARCH ORTHOP, 119(7-8), 1999, pp. 410-417
Citations number
57
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Ortopedics, Rehabilitation & Sport Medicine
Journal title
ARCHIVES OF ORTHOPAEDIC AND TRAUMA SURGERY
ISSN journal
0936-8051 → ACNP
Volume
119
Issue
7-8
Year of publication
1999
Pages
410 - 417
Database
ISI
SICI code
0936-8051(199911)119:7-8<410:BASTDA>2.0.ZU;2-3
Abstract
Disuse atrophy has been the subject of research studies of an animal model in which single-limb immobilization induces atrophic changes in the immobil ized limb. These reveal systemic changes in the experimental animals that g o far beyond the local response expected in that situation and are not full y understood as yet. We therefore performed a biochemical study on the effe ct of hind-limb immobilization on the serum and tissues of rats. The experi ment was carried out on 70 young Sprague-Dawley male rats. In one group of 35 rats, the left hind-limb was immobilized for 3 weeks. Another group of 3 5 rats served as controls. Serum total protein, albumin, urea, creatinine, and calcium were found to be reduced during immobilization. Serum cholester ol levels, on the other hand, increased to a considerable extent. No change s were recorded with phosphate, bilirubin, and magnesium. Serum alanine ami notransferase (ALT) and alkaline phosphatase (ALP) were both reduced in act ivity. The activity of muscle aspartate aminotransferase (AST) and bone alk aline phosphatase (ALP) was also decreased. Lactate dehydrogenase (LDH) rem ained unchanged in both serum and muscle. We discuss our findings in the li ght of previous knowledge regarding the atrophic process.