Modeling the interactions of particulates with epithelial lining fluid antioxidants

Citation
H. Zielinski et al., Modeling the interactions of particulates with epithelial lining fluid antioxidants, AM J P-LUNG, 277(4), 1999, pp. L719-L726
Citations number
30
Language
INGLESE
art.tipo
Article
Categorie Soggetti
da verificare
Journal title
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
ISSN journal
1040-0605 → ACNP
Volume
277
Issue
4
Year of publication
1999
Pages
L719 - L726
Database
ISI
SICI code
1040-0605(199910)277:4<L719:MTIOPW>2.0.ZU;2-D
Abstract
Oxidative stress may be a fundamental mode of injury associated with inspir ed particles. To examine this, we determined the ability of three carbon bl ack particles (CBPs; M120, M880, and R250) and two forms of silicon dioxide , amorphous (Cabosil) and crystalline (DQ12) quartz, to deplete epithelium lining fluid antioxidant defenses. Single and composite antioxidant solutio ns of uric acid, ascorbic acid (AA), and reduced glutathione (GSH) were exa mined in the presence of particle concentrations of 150 mu g/ml. Uric acid was not depleted by any particle considered. AA was depleted in a near-line ar fashion with time by the three different CBPs; however, AA depletion rat es varied markedly with CBP type and decreased in the presence of metal che lators. An initially high GSH depletion rate was noted with all CBPs, and t his was always accompanied by the appearance of oxidized glutathione. Expos ure to Cabosil or DQ12 did not result in the loss of GSH. Together, these d ata demonstrate that particle type, size, and surface area are all importan t factors when considering particle-antioxidant interactions in the airways .