Lack of association of a common polymorphism of the plasminogen activator inhibitor-1 gene with coronary artery disease and myocardial infarction

Citation
Jl. Anderson et al., Lack of association of a common polymorphism of the plasminogen activator inhibitor-1 gene with coronary artery disease and myocardial infarction, J AM COL C, 34(6), 1999, pp. 1778-1783
Citations number
34
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cardiovascular & Respiratory Systems","Cardiovascular & Hematology Research
Journal title
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY
ISSN journal
0735-1097 → ACNP
Volume
34
Issue
6
Year of publication
1999
Pages
1778 - 1783
Database
ISI
SICI code
0735-1097(19991115)34:6<1778:LOAOAC>2.0.ZU;2-S
Abstract
OBJECTIVES The study was done to assess whether the common polymorphic alle le (4G) of the plasminogen activator inhibitor-1 (PAI-1) gene is associated with coronary artery disease (CAD) or myocardial infarction (MI). BACKGROUND Impaired fibrinolytic function has been associated with CAD and MI. Plasminogen activator inhibitor-1 pla;vs a central role in intravascula r thrombosis and thrombolysis; the common insertion/deletion polymorphism ( 4G/5G) of PAI-1 has been correlated with altered PAI-1 levels and proposed as a coronary risk factor. METHODS Blood was drawn and DNA extracted from 1,353 consenting patients un dergoing coronary angiography. The 4G and 5G alleles of PAI-1 were amplifie d using specific primers. Amplified products were visualized by staining wi th ethidium bromide after electrophoresis in 1.5% ag-arose. RESULTS Patient age averaged 63.5 (SD 11.7) years; 70% were men, 28% had a history of MI, 66%, had severe CAD (>60% stenosis), and 23% had no CAD or M I. Overall, the frequency of the 4G allele was 54.2%, and 78% of patients w ere 4G carriers. Genotypic distributions were: 4G/4G = 30.1%, 4G/5G = 47.9% , and 5G/5G = 21.8%. Neither carriage of 4G (CAD odds ratio [OR] = 1.08 [0. 80 to 1.46], MI OR = 1.11 [0.83 to 1.49]) nor 4G/4G homozygosity (CAD OR = 1.07, MI OR = 0.98) was associated with CAD or MI. In multivariate analyses , risk factors associated with CAD were (in order): gender, age, smoking, d iabetes, cholesterol, and hypertension; for MI, they were gender, smoking, and cholesterol. CONCLUSIONS A common PAI-1 polymorphism (4G) was not importantly associated with angiographic CAD or history of MI in a Caucasian population. Modest r isk (i.e., OR <1.5), especially for MI, or risk in association with other f actors, cannot be excluded (C) 1999 by the American College of Cardiology.