A novel microtubule-associated protein-2 expressed in oligodendrocytes in multiple sclerosis lesions

Citation
B. Shafit-zagardo et al., A novel microtubule-associated protein-2 expressed in oligodendrocytes in multiple sclerosis lesions, J NEUROCHEM, 73(6), 1999, pp. 2531-2537
Citations number
22
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences & Behavoir
Journal title
JOURNAL OF NEUROCHEMISTRY
ISSN journal
0022-3042 → ACNP
Volume
73
Issue
6
Year of publication
1999
Pages
2531 - 2537
Database
ISI
SICI code
0022-3042(199912)73:6<2531:ANMPEI>2.0.ZU;2-N
Abstract
Elucidation of the mechanisms involved in the regeneration of oligodendrocy tes and remyelination is a central issue in multiple sclerosis (MS) researc h. We recently identified a novel alternatively spliced, developmentally re gulated oligodendrocyte-specific protein designated microtubule-associated protein-2 + 13 [microtubule-associated protein-2 expressing exon 13 (MAP-2 + 13)]. MAP-2 + 13 is expressed in human fetal oligodendrocytes during proc ess extension and myelination but is minimally expressed in normal mature C NS. To test the hypothesis that MAP-2+13 is reexpressed in regenerating oli godendrocytes in MS lesions, we examined the brains of MS patients for the expression of this protein. By immunocytochemistry using a series of monocl onal antibodies specific for MAP-2 + 13, we determined that MAP-2 + 13 expr ession was up-regulated in all 31 lesions from 10 different MS brains. MAP- 2 + 13 was expressed in regenerating oligodendrocytes associated with demye linated lesions, with the highest counts found in regions of extensive remy elination. By electron microscopy, MAP-2 + 13 was localized to oligodendroc ytes engaged in remyelination, evident by their process extension and assoc iation with thinly myelinated (remyelinated) and demyelinated axons. These results suggest a hitherto unsuspected role for this microtubule-associated protein in oligodendrocyte function during development and myelin repair.