A micronucleus assay in sea urchin embryos

Citation
K. Saotome et al., A micronucleus assay in sea urchin embryos, MUT RES-GTE, 446(1), 1999, pp. 121-127
Citations number
26
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Molecular Biology & Genetics
Journal title
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
ISSN journal
1383-5718 → ACNP
Volume
446
Issue
1
Year of publication
1999
Pages
121 - 127
Database
ISI
SICI code
1383-5718(19991029)446:1<121:AMAISU>2.0.ZU;2-Z
Abstract
We have developed a micronucleus assay for use in sea urchin embryos. The e mbryos at the early blastula stage (about 256 cells) were exposed to genoto xic chemicals overnight until control embryos have reached the gastrula sta ge. Then all embryos were suspended in 1 M urea, dissociated by pipetting, and fixed with methanol:acetic acid (9:1). The preparations were air-dried and stained with acridine orange. The test chemicals (mitomycin C [MMC], vi nblastine and 1-beta-D-arabinofuranosylcytosine [Ara C]) induced clear micr onuclei dose-dependently, The maximum frequency induced with MMC was 2-3% i n Clypeaster japonicus and 1-2% in Hemicentrotus pulcherrimus. (C) 1999 Pub lished by Elsevier Science B.V. All rights reserved.