EGFR family-mediated signal transduction in the human keratinocyte cell line HaCaT

Citation
Mm. Marques et al., EGFR family-mediated signal transduction in the human keratinocyte cell line HaCaT, EXP CELL RE, 252(2), 1999, pp. 432-438
Citations number
34
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell & Developmental Biology
Journal title
EXPERIMENTAL CELL RESEARCH
ISSN journal
0014-4827 → ACNP
Volume
252
Issue
2
Year of publication
1999
Pages
432 - 438
Database
ISI
SICI code
0014-4827(19991101)252:2<432:EFSTIT>2.0.ZU;2-B
Abstract
We analyzed the formation of homo- and heterodimers between EGFR, ErbB2, an d ErbB3 (members of the EGF receptor family) in the human skin keratinocyte cell Line HaCaT, in dependence of the added ligand. ErbB4 was not unambigu ously identified. By immunoprecipitation and Western blots, we showed the f ormation of heterodimers between all members of the family. Whereas EGF and TGF-alpha strongly induced heterodimerization, no effect was observed with heregulin. At the concentrations used all ligands elicited a similar, diff erentiation-independent activation of erk1/2 MAP kinase, with the exception of heregulin which activated p42/44 only marginally. We also found that di fferent ligands triggered different transcription patterns of "early genes, " with the exception of heregulin which did not, modulate transcription. TG F-alpha was the most efficient Ligand in promoting incorporation of tritiat ed thymidine into the DNA. (C) 1999 Academic Press.