Valacyclovir inhibits recovery of ocular HSV-1 after experimental reactivation by excimer laser keratectomy

Citation
Dk. Dhaliwal et al., Valacyclovir inhibits recovery of ocular HSV-1 after experimental reactivation by excimer laser keratectomy, CORNEA, 18(6), 1999, pp. 693-699
Citations number
18
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Optalmology
Journal title
CORNEA
ISSN journal
0277-3740 → ACNP
Volume
18
Issue
6
Year of publication
1999
Pages
693 - 699
Database
ISI
SICI code
0277-3740(199911)18:6<693:VIROOH>2.0.ZU;2-U
Abstract
Purpose. The goal of this was to determine whether the systemic administrat ion of valacyclovir (Valtrex) would reduce ocular shedding of herpes simple x virus 1 (HSV-1) after excimer laser ablation in the New Zealand rabbit la tency model. Methods. The in vitro 50% inhibitory concentration (IC50) of H SV-1 W strain was determined by using a plaque-reduction assay to verify it s sensitivity to acyclovir. Forty-seven NZW rabbits latently infected with HSV-1 W strain were divided into four groups: I, 50 mg/kg/day valacyclovir; II, 100 mg/kg/day valacyclovir; III, 150 mg/kg/day valacyclovir; and IV, s aline control. One half of the total dose of valacyclovir was delivered via intraperitoneal injections twice daily for 7 days beginning with one dose before excimer laser keratectomy. HSV-1 ocular shedding was determined from eye cultures for 7 days after treatment. Results. The IC50 for HSV-1 W was determined to be 2.9 mu g/ml. The administration of both 100 mg/kg/day (gr oup IT) and 150 mg/kg/day (group III) of valacyclovir significantly reduced the number of eyes from which latent HSV-1 was recovered compared with the control group. There was no difference between the control group and group I (50 mg/kg/day valacyclovir). However, all three valacyclovir dosages sig nificantly reduced the total number of HSV-1 shedding days compared with th e control group, and 100% HSV-1 TG latency was demonstrated for all four gr oups. Conclusion. Systemic administration of valacyclovir significantly red uced HSV-1 ocular shedding in a dose-dependent manner after excimer laser k eratectomy in the NZW rabbit latency model.