Studies of glomerular permeability factor (GPF) in focal segmental glomerular sclerosis and the relationship between GPF and vascular permeability factor (VPF)

Citation
S. Kondo et al., Studies of glomerular permeability factor (GPF) in focal segmental glomerular sclerosis and the relationship between GPF and vascular permeability factor (VPF), CLIN NEPHR, 52(5), 1999, pp. 278-284
Citations number
22
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Urology & Nephrology","da verificare
Journal title
CLINICAL NEPHROLOGY
ISSN journal
0301-0430 → ACNP
Volume
52
Issue
5
Year of publication
1999
Pages
278 - 284
Database
ISI
SICI code
0301-0430(199911)52:5<278:SOGPF(>2.0.ZU;2-C
Abstract
Background: We previously demonstrated that the supernatants of cultured co ncanavalin-A (con-A) stimulated peripheral blood mononuclear cells (PBMC) f rom patients with minimal change nephrotic syndrome (MCNS) increased the ur inary protein excretion in injected rats and suggested that PBMC released a factor, which we called glomerular permeability factor (GPF), changes in t he glomerular permeability and thus resulted in proteinuria in MCNS. Materi al and methods: Tn this study we investigated the GPF activity in focal seg mental glomerular sclerosis (FGS) and other conditions of chronic glomerulo nephritis (CGN), and also the relationship between GPF and vascular permeab ility factor (VPF). In experiment 1 the supernatants of the cultured con-A stimulated PBMC from patients with 10 FGS, 5 other CGN and 10 controls were tested regarding their ability to produce GPF. The GPF activity was define d as positive when the 8-hour urinary protein excretion after the injection of the supernatant in Sprague-Dawley rats exceeded the mean value plus 2 s tandard deviations (M + 2 SD) of that before injection. Results: Three out of 10 FGS patients and 1 membranous nephropathy patient out of the 5 other CGN patients were positive for GPF activity. In experiment 2 the relationsh ip between GPF and VPF was analyzed using culture supernatants of PBMC from 10 nephrotic MCNS patients and 15 controls. The VPF activity was measured following the method developed by Ovary [1975]. All 7 cases that were posit ive for GPF activity were simultaneously positive for VPF activity. On the other hand, 16 cases that were positive for VPF activity were not always po sitive for GPF activity (7 cases were positive and 9 were negative for VPF activity). Conclusion: Experiments 1 and 2 thus suggested that GPF was not active in MCNS alone, but also in other CGN conditions and it was therefore not considered to be the same factor/substance(s) as VPF.