A human IAP-family gene, Apollon, expressed in human brain cancer cells

Citation
Zh. Chen et al., A human IAP-family gene, Apollon, expressed in human brain cancer cells, BIOC BIOP R, 264(3), 1999, pp. 847-854
Citations number
32
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN journal
0006-291X → ACNP
Volume
264
Issue
3
Year of publication
1999
Pages
847 - 854
Database
ISI
SICI code
0006-291X(19991102)264:3<847:AHIGAE>2.0.ZU;2-F
Abstract
IAP is a family of protein that has baculovirus IAP repeat (BIR) domains an d inhibits apoptosis. We found a human IAP family gene, which we named Apol lon, encoding a huge protein (530 kDa) that contains a single BIR domain an d a ubiquitin-conjugating enzyme domain, that is a human homolog of BRUCE. Apollon protein was expressed in four of six brain cancers (gliomas), and o ne of five ovarian cancers in 38 human cancer cell lines that we examined. Among the brain cancer cell lines, SNB-78 expressed a high level of Apollon , and this cell line shows resistance against various anticancer drugs. Tre ating SNB-78 cells with antisense oligonucleotide against Apollon reduced t he expression of Apollon protein, and significantly sensitized the cells to apoptosis induced by cisplatin and camptothecin. These results suggest tha t Apollon protects SNB-78 cells from undergoing apoptosis and, at least in part, plays a role in tumorigenesis and drug resistance of this cell line. (C) 1999 Academic Press.