Tyrosine kinase participates in alpha(1A)-adrenoceptor-mediated increase of intracellular calcium in human embryo kidney 293 cells

Citation
Wz. Zhu et al., Tyrosine kinase participates in alpha(1A)-adrenoceptor-mediated increase of intracellular calcium in human embryo kidney 293 cells, ACT PHAR SI, 20(11), 1999, pp. 1025-1030
Citations number
17
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Pharmacology & Toxicology
Journal title
ACTA PHARMACOLOGICA SINICA
ISSN journal
0253-9756 → ACNP
Volume
20
Issue
11
Year of publication
1999
Pages
1025 - 1030
Database
ISI
SICI code
0253-9756(199911)20:11<1025:TKPIAI>2.0.ZU;2-Y
Abstract
AIM: To determine the role of protein-tyrosine kinase (PTK) in alpha(1A)-ad renoceptor-mediated increase of [Ca2+ ](i) (intracellular calcium) in human embryo kidney (HEK) 293 cells expressed alpha(1A)-adrenoceptor. METHODS: E ffects of two PTX inhibitors: genistein and tyrphostin, were investigated o n the increase of [Ca2+ ](i) by using Fura-2, The activity of PTK was measu red and the accumulation of [H-3]InsPs were observed. RESULTS: Norepinephri ne stimulated a rapid increase in [Ca2+ ](i) to (371 +/- 31) nmol . L-1 in HEK 293 cells. Norepinephrine-induced increase of [Ca2+ ](i) was inhibited by the tyrosine kinase inhibitors quercetin and tyrphostin by 23.8 % and 21 .4 %, respectively, but the accumulation of [H-3]InsPs induced by norepinep hrine was not. The activity of the plasma-associated tyrosine kinase was in creased to (1.73 +/- 0.72)-fold over the control by norepinephrine 10 mu mo l . L-1. The norepinephrine-activated PTK was inhibited by calphostin C and depletion of intra- and extra-cellular Ca2+. CONCLUSION: The PTK participa tes in mobilization of Ca2+ mediated by alpha(1A)-adrenoceptors in HEK 293 cell lines.