Identification of Enterobacteriaceae by partial sequencing of the gene encoding translation initiation factor 2

Citation
J. Hedegaard et al., Identification of Enterobacteriaceae by partial sequencing of the gene encoding translation initiation factor 2, INT J SY B, 49, 1999, pp. 1531-1538
Citations number
32
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
ISSN journal
0020-7713 → ACNP
Volume
49
Year of publication
1999
Part
4
Pages
1531 - 1538
Database
ISI
SICI code
0020-7713(199910)49:<1531:IOEBPS>2.0.ZU;2-A
Abstract
Nucleotide sequence analysis is increasingly being used to identify bacteri a. In this work, a PCR assay based on degenerate primers was used to obtain the partial sequence of infB, the gene encoding translation initiation fac tor 2 (IF2), in 39 clinical isolates of different Enterobacteriaceae. The p artial sequence encodes the GTP-binding domain of IF2. Together with sequen ces from the literature, a total of 15 species, each represented by one to seven strains, was investigated. Phylogenetic analysis yielded an evolution ary tree which had a topology similar to a tree constructed using available 16S rRNA sequences. It is concluded that the inter-species variation of th e infB gene fragment is sufficient for its use in the characterization of s trains that have aberrant phenotypic reactions.