Comparison of partial malolactic enzyme gene sequences for phylogenetic analysis of some lactic acid bacteria species and relationships with the malic enzyme

Citation
A. Groisillier et A. Lonvaud-funel, Comparison of partial malolactic enzyme gene sequences for phylogenetic analysis of some lactic acid bacteria species and relationships with the malic enzyme, INT J SY B, 49, 1999, pp. 1417-1428
Citations number
76
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
ISSN journal
0020-7713 → ACNP
Volume
49
Year of publication
1999
Part
4
Pages
1417 - 1428
Database
ISI
SICI code
0020-7713(199910)49:<1417:COPMEG>2.0.ZU;2-K
Abstract
DNA sequences covering 36% of the mle gene that encodes the malolactic enzy me were determined for 13 strains of lactic acid bacteria, representing Ped iococcus. Leuconostoc. Lactobacillus and Oenococcus genera. The sequences w ere aligned with the corresponding region of mleS in Lactococcus lactis. Th e phylogenetic distance matrix tree of all mle sequences was compared with the 16S rRNA phylogenetic tree. The analysis showed that the mle fragment e volved more rapidly than the 16S gene and differently. Pediococcus and Lact obacillus species were intermixed in the 16S rRNA tree whereas they were se parated in the mle tree. Leuconostoc mesenteroides and Oenococcus oeni were distinct from other species in the 16S rRNA tree, whereas they were interm ixed with Lactobacillus species and Lactococcus lactis in the mle tree. The amino acid sequences deduced from partial mle genes were aligned with 22 m alic enzyme sequences and the corresponding phylogenetic tree was construct ed. Malic and malolactic enzymes were distinct at the phylogenetic level, e xcept for malic enzymes of yeast and Escherichia coli which were nearer the malolactic enzymes than the other malic enzymes. The analysis of conserved sites showed several interesting amino acids specific to either malic enzy me or malolactic enzyme.