Determination of members of a Borrelia afzelii-related group isolated fromIxodes nipponensis in Korea as Borrelia valaisiana

Citation
T. Masuzawa et al., Determination of members of a Borrelia afzelii-related group isolated fromIxodes nipponensis in Korea as Borrelia valaisiana, INT J SY B, 49, 1999, pp. 1409-1415
Citations number
41
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
ISSN journal
0020-7713 → ACNP
Volume
49
Year of publication
1999
Part
4
Pages
1409 - 1415
Database
ISI
SICI code
0020-7713(199910)49:<1409:DOMOAB>2.0.ZU;2-#
Abstract
The 16S rRNA sequences of the Korean Borrelia strains 5MT and 9MT, isolated from Ixodes nipponensis, showed identities of 99.0-99.1% to that of B. afz elii. The strains were tentatively classified as belonging to the B. afzeli i-related group. In this study, Korean isolates, including these strains, w ere characterized further and compared with recently described new species. These strains generated a RFLP pattern that has not been found previously in RFLP analysis of the 5S-23S rRNA intergenic spacer and the flagellin gen e. When phylogenetic trees were constructed, based on the 5S-23S rRNA inter genic spacer, flagellin gene and 16S rRNA sequences, these Korean isolates formed a cluster with the Borrelia strain Am501 isolated from Ixodes column ae in Japan and Borrelia valaisiana strains VS116(T) and UK isolated from I xodes ricinus in Europe and were distinguishable from the other species. Ho wever, these three groups of strains were divergent from each other in the molecular masses of the putative outer surface protein A (OspA) and in the sequences of the ospA gene. These findings suggest that these Korean isolat es and one Japanese isolate are members of B. valaisiana and that OspA of t his species is divergent, as is that of Borrelia garinii. This led to the s peculation that B. valaisiana strains are adapted to the vector ticks found in each locality.