Psychrophilic sulfate-reducing bacteria isolated from permanently cold Arctic marine sediments: description of Desulfofrigrus oceanense gen. nov., spnov., Desulfofrigus fragile sp nov., Desulfofaba gelida gen. nov., sp nov., Desulfotalea psychrophila gen. nov., sp nov and Desulfotalea arctica sp nov.

Citation
C. Knoblauch et al., Psychrophilic sulfate-reducing bacteria isolated from permanently cold Arctic marine sediments: description of Desulfofrigrus oceanense gen. nov., spnov., Desulfofrigus fragile sp nov., Desulfofaba gelida gen. nov., sp nov., Desulfotalea psychrophila gen. nov., sp nov and Desulfotalea arctica sp nov., INT J SY B, 49, 1999, pp. 1631-1643
Citations number
37
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
ISSN journal
0020-7713 → ACNP
Volume
49
Year of publication
1999
Part
4
Pages
1631 - 1643
Database
ISI
SICI code
0020-7713(199910)49:<1631:PSBIFP>2.0.ZU;2-S
Abstract
Five psychrophilic, Gram-negative, sulfate-reducing bacteria were isolated from marine sediments off the coast of Svalbard. All isolates grew at the i n situ temperature of -1.7 degrees C. In batch cultures, strain PSv29(T) ha d the highest growth rate at 7 degrees C, strains ASv26(T) and LSv54(T) had the highest growth rate at 10 degrees C, and strains LSV21(T) and LSv514(T ) had the highest growth rate at 18 degrees C. The new isolates used the mo st common fermentation products in marine sediments, such as acetate, propi onate, butyrate, lactate and hydrogen, but only strain ASv26(T) was able to oxidize fatty acids completely to CO2. The new strains had growth optima a t neutral ph and marine salt concentration, except for LSv54(T) which grew fastest with 1% NaCl. Sulfite and thiosulfate were used as electron accepte rs by strains ASv26(T), PSv29(T) and LSv54(T), and all strains except PSv29 (T) grew with Fe3+ (ferric citrate) as electron acceptor. Chemotaxonomy bas ed on cellular fatty acid patterns and menaquinones showed good agreement w ith the phylogeny based on 16S rRNA sequences. All strains belonged to the delta subclass of Proteobacteria but had at least 9% evolutionary distance from known sulfate reducers. Due to the phylogenetic and phenotypic differe nces between the new isolates and their closest relatives, establishment of the new genera Desulfotalea gen. nov., Desulfofaba gen. nov. and Desulfofr igus gen. nov. is proposed, with strain ASv26(T) as the type strain of the type species Desulfofrigus oceanense sp. nov., LSV21(T) as the type strain of Desulfofrigus fragile sp. nov., PSv29(T) as the type strain of the type species Desulfofaba gelida sp. nov., LSv54(T) as the type strain of the typ e species Desulfotalea psychrophila sp. nov. and LSv514T as the type strain of Desulfotalea arctics sp. nov.